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ABSTRACT Introduction: In modern gymnastics, there are high demands for the physical quality of Chinese athletes. Objectives: This paper mainly studies whether the workload of Chinese gymnasts can support the corresponding high-intensity training in the training process. Methods: Experimental scientific research methods and statistical analysis are used to conduct a long-term study on dozens of gymnasts in Chinese schools and draw the workload curves of these gymnasts during gymnastic exercises. We try to determine the effective correlation between the athlete's body load and physical training and body shape. Results: During the training of gymnasts, heart rates can briefly exceed 190 beats per minute. Conclusion: In the training process of different gymnasts, the gymnasts' heart rates show obvious differences. Therefore, the use of scientific and reasonable training strategies can effectively improve the ability of athletes' hearts to withstand high-intensity exercise loads and help improve the gymnast's performance. Level of evidence II; Therapeutic studies - investigation of treatment results.
RESUMO Introdução: Há altas exigências para a qualidade física dos atletas chineses na ginástica moderna. Objetivos: Este trabalho tem como principal estudo verificar se a carga de trabalho dos ginastas chineses pode suportar o correspondente treinamento de alta intensidade no processo de treinamento. Métodos: Foram utilizados métodos de investigação científica experimental e análise estatística para efetuar um estudo de longo prazo sobre dezenas de ginastas nas escolas chinesas além de desenhar as curvas de carga de trabalho desses ginastas durante os exercícios. Tentamos determinar a correlação efetiva entre a carga de trabalho e o treinamento físico do atleta e sua forma corporal. Resultados: Durante o treinamento de alta intensidade dos ginastas chineses, os batimentos cardíacos puderam exceder brevemente os 190 batimentos por minuto. Conclusão: No processo de treinamento de diferentes ginastas, os batimentos cardíacos dos ginastas apresentam diferenças óbvias. Portanto, o uso de estratégias de treinamento científicas e razoáveis pode efetivamente melhorar a capacidade do coração dos atletas afim de suportar cargas de exercício de alta intensidade e ajudar a melhorar o desempenho do ginasta. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción: La calidad física de los atletas chinos en la gimnasia moderna es muy exigente. Objetivos: El estudio principal de este trabajo es comprobar si la carga de trabajo de las gimnastas chinas puede soportar el correspondiente entrenamiento de alta intensidad en el proceso de entrenamiento. Métodos: Se utilizaron métodos de investigación científica experimental y análisis estadísticos para llevar a cabo un estudio a largo plazo sobre docenas de gimnastas de escuelas chinas, además de dibujar las curvas de carga de trabajo de estas gimnastas durante los ejercicios. Intentamos determinar la correlación efectiva entre la carga de trabajo y el entrenamiento físico y la forma del cuerpo del atleta. Resultados: Durante el entrenamiento de alta intensidad de las gimnastas chinas, las frecuencias cardíacas podían superar brevemente las 190 pulsaciones por minuto. Conclusión: En el proceso de entrenamiento de diferentes gimnastas, las frecuencias cardíacas de las gimnastas muestran diferencias evidentes. Por lo tanto, el uso de estrategias de entrenamiento científicas y razonables puede mejorar eficazmente la capacidad cardíaca de las atletas para soportar cargas de ejercicio de alta intensidad y ayudar a mejorar el rendimiento del gimnasta. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
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ABSTRACT Objectives Evaluate the application value of 3D printing technology in measuring acetabular bone defect area in adult patients diagnosed with developmental dysplasia of the hip (DDH). Methods 23 cases of DDH requiring total hip replacement surgery were enrolled in this study. Preoperative examination confirmed the standard pelvic plain films Crowe, including 3 cases of Crowe I, 7 Crowe II, and 13 Crowe III. The 3D printing technology was used to print the hip model before the operation. Based on the pre-printed model, pre-operative planning and surgical procedures were established. The area of the acetabular bone defects was measured, the selected size prosthesis was recorded, and the surgery was performed (group A). The actual acetabular bone defect area and the prosthesis size were also recorded (group B). Results The comparative results indicated that the actual acetabular defect area measured intraoperatively and the area measured using the 3D printing technology did not significantly differ for all participants (all P>0.05). Conclusion Preoperative model can accurately measure the acetabular bone defect area for DDH. It is significant to develop individualized implants for DDH patients treated with the 3D printing technique. Level of Evidence IV: Case series.
RESUMO Objetivos Avaliar o potencial da aplicação da tecnologia de impressão 3D na medição da área de defeito ósseo acetabular em pacientes adultos diagnosticados com displasia do desenvolvimento do quadril (DDH). Métodos 23 casos de DDH que requereram cirurgia de substituição total do quadril foram incluídos neste estudo. O exame pré-operatório confirmou os filmes pélvicos padrão Crowe, incluindo 3 casos de Crowe I, 7 Crowe II, e 13 Crowe III. A tecnologia de impressão 3D foi utilizada para imprimir o modelo de quadril antes da operação. Com base no modelo pré-impresso, o planejamento pré-operatório e os procedimentos cirúrgicos foram estabelecidos. A área dos defeitos ósseos acetabulares foi medida, a prótese de tamanho selecionado foi registrada, e a cirurgia foi realizada (grupo A). A área do defeito ósseo acetabular real e o tamanho da prótese também foram registrados (grupo B). Resultados Os resultados comparativos indicaram que a área real do defeito acetabular medida intraoperativamente e a área medida usando a tecnologia de impressão 3D não diferiu significativamente para todos os participantes (todos P>0,05). Conclusão O modelo pré-operatório pode medir com precisão a área de defeito ósseo acetabular para DDH. É relevante desenvolver implantes individualizados para pacientes com DDH tratados com a técnica de impressão 3D. Nível de Evidência IV: Série de casos.
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This paper explores the potential mechanism of microRNA-143–5p regulation effects on pulmonary arterysmooth muscle cells (PASMCs) functions in hypoxic pulmonary hypertension (HPH) via targeting HIF-1a,which may offer a new idea for HPH therapy. PASMCs were transfected with mimics control/miR-143–5pmimics or inhibitor control/miR-143–5p inhibitor. We used Western blotting and RT-qPCR to detect the proteinand mRNA expressions, CCK-8 assay to detect cellular viability, Annexin V-FITC/PI staining and caspase3/cleaved caspase-3 protein to evaluate cellular apoptosis, transwell migration experiment for cellularmigration measurement and Dual luciferase reporter gene assay to prove the target of miR-143–5p. Cells underhypoxic condition presented the decreased protein and mRNA expressions of a-smooth muscle actin (SM-aactin), Myocardin, smooth muscle myosin heavy chain (SMMHC), and smooth muscle-22a (SM22a),Calponin1 and Hypoxia-inducible factor-1a(HIF-1a), the increased cell viability and miR-143–5p level; Overexpression of miR-143–5p obviously reduced vascular smooth muscle-specific contraction marker proteinlevels and cellular apoptosis, increased cellular migration of PASMCs with hypoxia stimulation; Low-expression of miR-143–5p caused the opposite changes, while co-transfected with Si HIF-1a blocked thebeneficial effects of miR-143–5p inhibition on PASMCs under hypoxia. MicroRNA-143–5p can promote thephenotype conversion, proliferation and migration of pulmonary artery smooth muscle cells under hypoxiccondition through direct targeting of HIF-1a.
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@#[Abstract] Objective: To investigate the effects of miR-625 and Resistin on the proliferation, invasion and migration of NSCLC cells as well as the growth of transplanted tumors in nude mice and their possible mechanisms. Methods: qPCR was used to detect the expression of miR-625 and Resistin in 80 pairs of NSCLC and corresponding para-cancerous tissues (specimens collected from NSCLC patients who were surgically treated in Xinjiang Uygur Autonomous Region People’s Hospital from March 2018 to October 2019) and four cell lines. Bioinformatics was adopted to predict the targeting relationship between miR-625 and Resistin, which was then verified by Dual luciferase gene reporter experiment. Overexpression or inhibition of miR-625 and Resistin in NSCLC cells was achieved with lipofection transfection technology, and the experimental cells were divided into miR-625 mimic group, miR-625 inhibitor group, si-Resisitin group, miR-625 inhibitor+si-Resisitin group and NC group. The effects of miR-625 and Resistin on proliferation, invasion and migration of NSCLC cells were detected by CCK-8, Transwell and Scratch test, respectively. Western blotting was used to detect the effects of miR-625 and Resistin on the expressions of PI3K/AKT/Snail pathway proteins related with EMT in NSCLC cells. A549 cell transplanted tumor model was constructed in nude mice to observe the effect of miR-625 and Resistin on the growth of xenografts. Results: Compared with para-cancerous tissues, miR-625 showed low expression while Resistin showed high expression in NSCLC tissues and four cell lines (both P<0.01), and the two were negatively correlated (r=-0.7183,P<0.01). The expression of Resistin was related to the degree of NSCLC differentiation, clinical stage and lymph node metastasis. Resistin was a target gene of miR-625. Compared with the Blank group and NC group, the proliferation, invasion and migration of NSCLC cell linesA549 and H226, as well as the growth of transplanted tumors in nude mice in the miR-625 mimic group and the si-Resistin group were significantly reduced (all P<0.05), while those indicators in the miR-625 inhibitor group were significantly improved (all P<0.05); However, co-transfection of miR-625 inhibitor and si-Resistin significantly reversed the effect of miR-625 inhibitor on above indicators (all P<0.05);And there was no significant difference between NC group and miR-625 inhibitor+si-Resistin group (all P >0.05). The protein expressions of p-AKT, p-PI3K, Snail, Twist1 and Vimentin also showed the same trend (all P<0.05), while the expression of E-cadherin protein changed in the opposite direction (P<0.05). Conclusion: miR-625 is lowly expressed in NSCLC tissues and cell lines, which can negatively regulate Resistin expression to inhibit the proliferation, invasion and migration of NSCLC cells and the growth of transplanted tumors in nude mice. The mechanism may be related to the PI3K/AKT/Snail signaling pathway.
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@#Warfarin is one of the most frequently prescribed oral anticoagulant. Many researches have shown that the genotypes have been strongly associated with warfarin maintenance doses. Especially, it has been accepted in academia that cytochrome P450 2C9 (CYP2C9) and vitamin K epoxide reductase complex 1 subunit (VKORC1) could affect the warfarin maintenance doses. There are also many other genotypes that were reported to be related to warfarin doses, but the results have been in controversial so far. The studies found that the dose formula which contained the genetic factors and clinical information could accurately predict the maintenance dose of warfarin, however, its usefulness is suspected due to the inconsistent results of clinical trials. Large-sample and multi-center studies are necessary to verify the specific effects of gene and non-gene factors to warfarin dose; at the same time, testing constructed models or building new models help to improve the explained percentages of individual differences.
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Objective?To investigate the application effect of laparoscopic exploration in laparoscopic percutaneous extaperitoneal closure (LPEC) and its value in preventing contralateral metachronous hernia (MCH).?Methods?The clinical data of 300 children with unilateral inguinal hernia treated by open herniorrhaphy (control group) and 200 children with unilateral inguinal hernia treated by LPEC (observation group) were analyzed retrospectively. In the observation group, the condition of contralateral groin was also explored by laparoscopy, and ligation of contralateral patent processus vaginalis (CPPV) was performed while in the control group, there was no exploration during operation. The operation indexes, postoperative complications and the incidence of MCH were compared between groups. The relationships between the occurrence of MCH and CPPV, age was analyzed.?Results?The postoperative pain visual analogue scale (VAS) score, postoperative ambulation time and hospitalization time of the observation group were significantly better than those of the control group (P < 0.05). There was no significant difference between the two groups in operation time and the total incidence of postoperative complications (P > 0.05). The incidence of postoperative MCH in the observation group was significantly lower than that in the control group (1.00% vs 4.67%) (P < 0.05). In the observation group, there were 72 cases of CPPV, the incidence rate of CPPV was 36.00%, and the incidence rate was related to age (P < 0.05). The MCH/CPPV risk ratio (RR) was 0.03, and 1/RR was about 33. Among all of the children, children with MCH and younger than 2 to 4 years old accounted for 43.75% while children of 0.5 to 2 years old accounted for 31.25%.?Conclusion?Compared with open herniorrhaphy, laparoscopic LPEC has characteristics of mild postoperative pain and faster recovery in the treatment of unilateral inguinal hernia, and it can significantly reduce the incidence of MCH. Laparoscopic exploration can accurately find CPPV. Ligation can reduce the incidence of MCH but it may cause excessive medical treatment.
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Endoscopic therapy (ET) is most common method for preventing variceal bleeding in cirrhosis,but the outcomes are not perfect.Recently,transjugular intrahepatic portosystemic shunt (TIPS) is introduced into clinical practice.However,the beneficial effects of TIPS compared to ET on cirrhotic patients is unknown.The aim of this study was to evaluate and compare the effects of TIPS with those of the most frequently used ET for prevention of variceal rebleeding (VRB) in liver cirrhosis.The PubMed,EMBASE,and Cochrane Library databases were searched from inception to February 2017.The primary study outcomes included the incidence of VRB,all-cause mortality,bleeding-related death,and the incidence of post-treatment hepatic encephalopathy (PTE).The odds ratios (ORs) with 95％ confidence intervals (CI) were pooled for dichotomous variables.Subgroup analyses were performed.Twenty-four studies were eligible and they included 1120 subjects treated with TIPS and 1065 subjects treated with ET.Although there was no significant difference in survival and PTE,TIPS was superior to ET in decreasing the incidence of VRB (OR=0.27;95％ CI,0.19-0.39,P＜0.00001),and decreasing the incidence of bleeding-related death (OR=0.21;95％ CI,0.13-0.32,P＜0.00001).Subgroup analysis found a lower mortality (OR=0.48;95％ CI,0.23-0.97;P=0.04) without any increased incidence of PTE (OR=1.37;95％ CI,0.75-2.50;P=0.31) in the studies of a greater proportion (≥40％) of patients with Child-Pugh class C cirrhosis receiving TIPS,and TIPS with covered stent did not increase the risk of PTE compared to ET (OR=1.52,95％ CI =0.82-2.80,P=0.18).It was concluded that TIPS with covered stent might be considered the preferred choice of therapy in patients with severe liver disease for secondary prophylaxis.
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Lupus peritonitis (LP) is extremely rare. Acute LP is characterized by rapid onset of ascites and severe abdominal pain, in addition to other well-recognized clinical features of a general systemic lupus erythematosus (SLE) fare. Ascites associated with acute LP has been rarely reported as the prominent feature of a SLE fare. We report a 39-year-old woman who developed massive, painful ascites as the presenting manifestation of a SLE fare. Diagnostic workup ruled out the possibility of hepatic, cardiovascular, infectious, or malignant diseases, and confirmed the presence of a SLE fare. The patient was treated with methyl prednisolone and hydroxychloroquine resulting in dramatic improvement of her condition. During ambulatory follow up, she has remained asymptomatic up to the moment of this writing. Adrenal steroids and hydrocychloroquine may be useful for the management of SLE fares in patients with massive, painful ascites due to acute LP.
La peritonitis lúpica es rara. El cuadro agudo se caracteriza por ascitis de comienzo rápido, dolor abdominal severo y otras manifestaciones de una recidiva de un lupus eritematoso sistémico (LES). Sólo ocasionalmente e ha informado que la ascitis y peritonitis lúpica sean las principales manifestaciones de una recidiva lúpica. Presentamos a una mujer de 39 años que presentó ascitis masiva y dolorosa como la primera manifestación de una reactivación lúpica. El estudio diagnóstico descartó problemas hepáticos, pulmonares, cardiacos, cáncer o infecciones y confirmó la reactivación lúpica. La paciente se trató con metilprednisolona y cloroquina, resultando en una mejoría dramática. Al momento de preparar este informe, la paciente permanece asintomática en control ambulatorio. Los corticoides y cloroquina pueden ser medicamentos útiles para el tratamiento de pacientes con ascitis dolorosa y masiva secundaria a peritonitis lúpica.
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Adult , Female , Humans , Ascites/etiology , Lupus Erythematosus, Systemic/complications , Peritonitis/etiology , Ascites/diagnosis , Peritonitis/diagnosis , Tomography, X-Ray ComputedABSTRACT
Purpose Evidence shows that adenosine triphosphate (ATP) is involved in the transmission of multiple chronic pain via P2X7 receptor. This study was to investigate the P2X7 and microglial cells in the chronic prostatitis pain. Materials and Methods Rats were divided into control group and chronic prostatitis group (n = 24 per group). A chronic prostatitis animal model was established by injecting complete Freund's adjuvant (CFA) to the prostate of rats, and the thermal withdrawal latency (TWL) was detected on days 0, 4, 12 and 24 (n = 6 at each time point in each group). Animals were sacrificed and the pathological examination of the prostate, detection of mRNA expression of P2X7 and ionized calcium binding adaptor molecule 1 (IBA-1) and measurement of content of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the dorsal horn of L5-S2 spinal cord were performed on days 0, 4, 12 and 24. In addition, the content of TNF-α and IL-1β in the dorsal horn of L5-S2 spinal cord was measured after intrathecal injection of inhibitors of microglial cells and/or P2X7 for 5 days. Results The chronic prostatitis was confirmed by pathological examination. The expression of P2X7 and IBA-1 and the content of TNF-α and IL-1β in rats with chronic prostatitis were significantly higher than those in the control group. On day 4, the expressions of pro-inflammatory cytokines became to increase, reaching a maximal level on day 12 and started to reduce on day 24, but remained higher than that in the control group. Following suppression of microglial cells and P2X7 receptor, the secretion of TNF-α and IL-1β was markedly reduced. Conclusion In chronic prostatitis pain, the microglial cells and P2X7 receptor are activated resulting in the increased expression of TNF-α and IL-1β in the L5-S2 spinal cord, which might attribute to the maintenance and intensification of pain in chronic prostatitis. .